Hyperbaric oxygen therapy attenuated limb ischemia in mice with high-fat diet by restoring Sirtuin 1 and mitochondrial function.

What Researchers Did

Researchers investigated the effects of hyperbaric oxygen therapy (HBO) on limb ischaemia in high-fat diet-fed mice and on human endothelial cells, focusing on sirtuin 1 (SIRT1) activation.

What They Found

HBO improved blood flow, enhanced vascular density, and reduced apoptosis in ischaemic calf muscles of high-fat diet-fed mice. In vitro, HBO restored cell proliferation, migration, and tube formation in human umbilical vein endothelial cells, reducing mitochondrial dysfunction and reactive oxygen species. These benefits were reversed by a SIRT1 inhibitor, indicating HBO's effects are primarily through SIRT1 activation.

Canadian Relevance

There is no direct Canadian connection mentioned in this study.

Study Limitations

The study was conducted in mouse models and in vitro cell cultures, which may not fully translate to human clinical outcomes.